Screening for host proteins interacting with Escherichia coli O157:H7 EspF

Enterohemorrhagic Escherichia coli (EHEC) is known to cause sporadic outbreaks of illness, including diarrhea, hemorrhagic colitis and hemolytic–uremic syndrome. Previous research has primarily focused on the development of attaching and effacing intestinal lesions and the secretion of Shiga toxin.

The study highlighted focuses on E. coli protein EspF, which is understood to participate in a number of processes damaging to host cells, including disruption of tight junctions and cytoskeletal rearrangements. However, the mechanism by which these damaging processes occur and the interactions of EspFare as yet unclear.

This research, published in Future Microbiology, screens host proteins for those that interact with EspF via flow cytometry and high-throughput screening to try and shed light on how EspF affects host cells to induce colitis and even colorectal carcinogenesis.

“EspF is well recognized as a significant effector protein, but little research has been done on its interaction with host proteins. The identification of bacteria–host interactions is therefore crucial in elucidating the pathogenic mechanism.”

Read the full article in Future Microbiology



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